Immortalization of human osteoblasts by transferring human telomerase reverse transcriptase gene.

@article{Xiaoxue2004ImmortalizationOH,
  title={Immortalization of human osteoblasts by transferring human telomerase reverse transcriptase gene.},
  author={Yin Xiaoxue and Chen Zhongqiang and Guo Zhaoqing and Dang Gengting and Ma Qingjun and Wang Shenwu},
  journal={Biochemical and biophysical research communications},
  year={2004},
  volume={315 3},
  pages={643-51}
}
In the current study, in order to establish an immortalized osteoblast cell line, human mesenchymal stem cells (hMSCs) had been inducted into osteoblasts directionally by an osteo-inductive conditioned medium, then the osteoblasts were steadily transduced by a retroviral vector containing human telomerase reverse transcriptase (hTERT) gene. The expression of hTERT, the telomerase activity, the telomere lengths, the tumorigenesis and the osteogenesis characteristics of transduced cells at… CONTINUE READING

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