Immortalization of human adult normal prostatic epithelial cells by liposomes containing large T-SV40 gene.

@article{Cussenot1991ImmortalizationOH,
  title={Immortalization of human adult normal prostatic epithelial cells by liposomes containing large T-SV40 gene.},
  author={O. Cussenot and P. Berthon and R. Berger and I. Mowszowicz and A. Faille and F. Hojman and P. Teillac and A. Le Duc and F. Calvo},
  journal={The Journal of urology},
  year={1991},
  volume={146 3},
  pages={
          881-6
        }
}
Simian virus SV40 has been widely used to immortalize epithelial cells of mammalian origin. We report here, for the first time to our knowledge, the immortalization of normal adult prostatic epithelial cells in culture by transfection of a plasmid containing SV40 genome with a defective replication origin (SV40 ori-) encapsulated into liposomes. These cells (PNT1) have now been cultured for more than 12 months, and shown to contain the SV40 genome. They express large T protein, present the… Expand
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