Immortality of cell lines: challenges and advantages of establishment

  title={Immortality of cell lines: challenges and advantages of establishment},
  author={Muhammad Irfan Maqsood and Maryam M. Matin and Ahmad Reza Bahrami and Mohammad M Ghasroldasht},
  journal={Cell Biology International},
Cellular immortality happens upon impairment of cell‐cycle checkpoint pathways (p53/p16/pRb), reactivation or up‐regulation of telomerase enzyme, or upregulation of some oncogenes or oncoproteins leading to a higher rate of cell division.There are also some other factors and mechanisms involved in immortalisation, which need to be discovered. Immortalisation of cells derived from different sources and establishment of immortal cell lines has proven useful in understanding the molecular pathways… 

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An analysis of a cluster of articles from the Embryo Project Encyclopedia provides information to suggest possible solutions to some potential problems in cell culturing, recognition of benefits for existing or historical culturing practices, and identification of gaps in scientific knowledge that warrant further research.

Evidence for immortality and autonomy in animal cancer models is often not provided, which causes confusion on key issues of cancer biology

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Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw

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Senescence and immortalization: role of telomeres and telomerase.

It is argued that telomere shortening in the absence of other alterations may be a potent tumor suppressor mechanism and the evidence for and against the major molecular mechanisms proposed to initiate replicative senescence are discussed.

Termination of lifespan of SV40‐transformed human fibroblasts in crisis is due to apoptosis

The model whereby SV40‐transformed cells acquire increased sensitivity to apoptosis based on changes in properties which activate caspase 8 in addition to changes previously described involving shortening of telomeric sequences is suggested.

Immortalization of Human Neural Stem Cells with the c-Myc Mutant T58A

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Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells

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Absence of cancer–associated changes in human fibroblasts immortalized with telomerase

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Differentiated mammalian cell lines immortalized by temperature-sensitive tumor viruses.

  • J. Chou
  • Biology
    Molecular endocrinology
  • 1989
A major goal of cell biologists is to have an in vitro cell model that simulates in vivo conditions. This cell model should be able to propagate in culture, express specialized tissue functions, and

Novel ARF/p53-independent senescence pathways in cancer repression

A major hurdle for p53 targeting in cancer treatment arises from the frequent deficiency or mutation of ARF or p53 in human cancers, which, in turn, profoundly compromises their tumor-suppressive ability.