Immortality of cell lines: challenges and advantages of establishment

@article{IrfanMaqsood2013ImmortalityOC,
  title={Immortality of cell lines: challenges and advantages of establishment},
  author={Muhammad Irfan Maqsood and Maryam M. Matin and Ahmad Reza Bahrami and Mohammad M Ghasroldasht},
  journal={Cell Biology International},
  year={2013},
  volume={37}
}
Cellular immortality happens upon impairment of cell‐cycle checkpoint pathways (p53/p16/pRb), reactivation or up‐regulation of telomerase enzyme, or upregulation of some oncogenes or oncoproteins leading to a higher rate of cell division.There are also some other factors and mechanisms involved in immortalisation, which need to be discovered. Immortalisation of cells derived from different sources and establishment of immortal cell lines has proven useful in understanding the molecular pathways… 

A History of Cellular Senescence and Its Relation to Stem Cells in the Twentieth and Twenty-First Centuries

TLDR
An analysis of a cluster of articles from the Embryo Project Encyclopedia provides information to suggest possible solutions to some potential problems in cell culturing, recognition of benefits for existing or historical culturing practices, and identification of gaps in scientific knowledge that warrant further research.

Evidence for immortality and autonomy in animal cancer models is often not provided, which causes confusion on key issues of cancer biology

TLDR
It is extrapolate that immortality and autonomy are established early during sporadic human carcinogenesis, unlike the late establishment in most animal models, and resumes many forerunners' work by determining the genetic bases for initiation, promotion and progression.

Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw

TLDR
Together, HaSKpwC7 and HaCaT cells represent two spontaneous (non-genetically engineered) “premalignant” keratinocyte lines from adult human skin that display different stages of the multistep process of skin carcinogenesis and thus represent unique models for analysing skin cancer development and progression.

Expansion of functional personalized cells with specific transgene combinations

TLDR
A new strategy for rapid cell expansion that overcomes current limitations is presented, and gene cocktails to expand primary cells from a variety of different tissues and species are developed, showing that expanded endothelial and hepatic cells retain properties of the differentiated phenotype.

Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1

TLDR
PUMC-CRC1 was a newly established and well characterized human colon cancer cell line, which might be a good model for both in vitro and in vivo studies of the mechanism of colon cancer progression and the treatment strategies for MSS CRC.

Establishment and transcriptomic features of an immortalized hepatic cell line of the Chinese tree shrew.

TLDR
An immortalized tree shrew hepatic cell line was established by introducing the simian virus 40 large T antigen gene into primary tree shrew hepatocytes, and it was found that the DNA replication- and cell cycle-related genes were upregulated, whereas the metabolic pathway- related genes were downregulated in early passages of immortalized hepatocytes compared to the PTHs.

Immortalizing Cells for Human Consumption

TLDR
C cultured meat cell lines will need to be approved as safe for consumption as food, proliferate and differentiate efficiently at industrial scales, and have desirable taste, texture, and nutrition characteristics for consumers.

Recent advances in conditional cell immortalization technology

TLDR
Key technologies used to create conditionally immortalized cells that can be expanded to clinical quantities in a stable and consistent fashion, yet can be returned to a normal, non-dividing state for therapeutic delivery to the patient are discussed.
...

References

SHOWING 1-10 OF 78 REFERENCES

Senescence induction; a possible cancer therapy

TLDR
By using high throughput genetic screening to search for genes involved in senescence, several candidate oncogenes and putative tumor suppressor genes have been recently isolated, including subtypes of micro-RNAs, which offer new perspectives in the modulation ofsenescence and open new approaches for cancer therapy.

Immortalization and transformation of primary human airway epithelial cells by gene transfer

TLDR
These immortalized human airways epithelial cells, which have been created through the stepwise introduction of genetic alterations, provide a novel experimental model system with which to study further the biology of the airway epithelial cell and to dissect the molecular basis of lung cancer pathogenesis.

Senescence and immortalization: role of telomeres and telomerase.

TLDR
It is argued that telomere shortening in the absence of other alterations may be a potent tumor suppressor mechanism and the evidence for and against the major molecular mechanisms proposed to initiate replicative senescence are discussed.

Termination of lifespan of SV40‐transformed human fibroblasts in crisis is due to apoptosis

TLDR
The model whereby SV40‐transformed cells acquire increased sensitivity to apoptosis based on changes in properties which activate caspase 8 in addition to changes previously described involving shortening of telomeric sequences is suggested.

Immortalization of Human Neural Stem Cells with the c-Myc Mutant T58A

TLDR
This work describes a novel and continuous cell line of immortalized human multipotent neural stem cells, in which the immortalizing agent is represented by a single gene which, in turn, carries a single and well characterized mutation.

Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells

TLDR
The results indicate that arrest of telomere shortening may be important in human papillomavirus-associated immortalization and that restoration ofTelomere length may be advantageous to cells with regard to their ability to proliferate.

A Novel, Immortal, and Multipotent Human Neural Stem Cell Line Generating Functional Neurons and Oligodendrocytes

TLDR
An immortalized hN SC line endowed with the properties of normal hNSCs is described, suitable for developing the novel, reliable assays and reproducible high‐throughput gene and drug screening that are essential in both diagnostics and cell therapy studies.

Absence of cancer–associated changes in human fibroblasts immortalized with telomerase

TLDR
Although ectopic expression of telomerase in human fibroblasts is sufficient for immortalization, it does not result in changes typically associated with malignant transformation, indicating that telomere shortening regulates the timing of cellular senescence.

Differentiated mammalian cell lines immortalized by temperature-sensitive tumor viruses.

  • J. Chou
  • Biology
    Molecular endocrinology
  • 1989
A major goal of cell biologists is to have an in vitro cell model that simulates in vivo conditions. This cell model should be able to propagate in culture, express specialized tissue functions, and

Novel ARF/p53-independent senescence pathways in cancer repression

TLDR
A major hurdle for p53 targeting in cancer treatment arises from the frequent deficiency or mutation of ARF or p53 in human cancers, which, in turn, profoundly compromises their tumor-suppressive ability.
...