Immobilized trypsin systems coupled on-line to separation methods: recent developments and analytical applications.

@article{Massolini2005ImmobilizedTS,
  title={Immobilized trypsin systems coupled on-line to separation methods: recent developments and analytical applications.},
  author={Gabriella Massolini and Enrica Calleri},
  journal={Journal of separation science},
  year={2005},
  volume={28 1},
  pages={7-21}
}
The ability to rapidly and efficiently digest and identify an unknown protein is of great utility for proteome studies. Identification of proteins via peptide mapping is generally accomplished through proteolytic digestion with enzymes such as trypsin. Limitations of this approach consist in manual sample manipulation steps and extended reaction times for proteolytic digestion. The use of immobilized trypsin for cleavage of proteins is advantageous in comparison with application of its soluble… CONTINUE READING

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