Immobilized pH gradients for isoelectric focusing. III. Preparative separations in highly diluted gels.

@article{Righetti1984ImmobilizedPG,
  title={Immobilized pH gradients for isoelectric focusing. III. Preparative separations in highly diluted gels.},
  author={Pier Giorgio Righetti and Cecilia Gelfi},
  journal={Journal of biochemical and biophysical methods},
  year={1984},
  volume={9 2},
  pages={103-19}
}
A further improvement on the preparative aspects of immobilized pH gradients (IPG) (J. Biochem. Biophys. Methods (1983) 8, 135-155, 157-172) is described, based on the use of soft (highly diluted) polyacrylamide gels. While in conventional IPGs in 5%T gels an upper load limit of 40-45 mg protein/ml gel volume is found, in 2.5%T gels, containing the same amount of Immobiline, as much as 90 mg protein/ml gel can be applied, without overloading effects. This is an extraordinary amount of material… CONTINUE READING

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