Immobilized DNA hairpins for assay of sequential breaking and joining of DNA backbones.

@article{Scott2006ImmobilizedDH,
  title={Immobilized DNA hairpins for assay of sequential breaking and joining of DNA backbones.},
  author={Benjamin O S Scott and Manuel Lavesa-Curto and Desmond R Bullard and Julea N Butt and Richard P Bowater},
  journal={Analytical biochemistry},
  year={2006},
  volume={358 1},
  pages={90-8}
}
Immobilized DNA hairpins are exploited in a novel approach to assay DNA ligases and nucleases. A fundamental characteristic of the assay is that a fluorophore at the remote terminus of the hairpin reports on the integrity of the DNA backbone. The functionality of the protocol is confirmed using ATP- and NAD+-dependent DNA ligases and the nicking enzyme N… CONTINUE READING