Immobilization-stabilization of penicillin G acylase from Escherichia coli.

  title={Immobilization-stabilization of penicillin G acylase from Escherichia coli.},
  author={Giuseppe Alvaro and Roberto Fernandez-Lafuente and Rosa Mar{\'i}a Blanco and Jos{\'e} M Guis{\'a}n},
  journal={Applied biochemistry and biotechnology},
  volume={26 2},
We have developed a strategy for immobilization-stabilization of penicillin G acylase from E. coli, PGA, by multipoint covalent attachment to agarose (aldehyde) gels. We hve studied the role of three main variables that control the intensity of these enzyme-support multiinteraction processes: 1. surface density of aldehyde groups in the activated support; 2. temperature; and 3. contact-time between the immobilized enzyme and the activated support prior to borohydride reduction of the… CONTINUE READING

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