Immobilization of recombinant thermostable beta-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk.

A recombinant thermostable beta-galactosidase from Bacillus stearothermophilus was immobilized onto chitosan using Tris(hydroxymethyl)phosphine (THP) and glutaraldehyde, and a packed bed reactor was utilized to hydrolyze lactose in milk. The thermostability and enzyme activity of THP-immobilized beta-galactosidase during storage was superior to that of free… CONTINUE READING