Immobilization of factor XIII on collagen membranes.

  title={Immobilization of factor XIII on collagen membranes.},
  author={B G Blanchy and Pierre R. Coulet and D. C. Gautheron},
  journal={Journal of biomedical materials research},
  volume={20 4},
Factor XIII from placenta was successfully grafted onto collagen membranes by the acyl-azide procedure. The transamidase activity retained on collagen membranes was determined by measuring the increase of fluorescence resulting from dansylcadaverine incorporation into casein. We studied the effect of different factors on the grafting: concentration and composition of the factor XIII preparation in the coupling solution and influence of the preactivation of factor XIII by thrombin. Stability… CONTINUE READING