Immobilization of cholesterol esterase and cholesterol oxidase onto sol-gel films for application to cholesterol biosensor.

Abstract

Cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) have been covalently immobilized onto tetraethylorthosilicate (TEOS) sol-gel films. The tetraethylorthosilicate sol-gel/ChEt/ChOx enzyme films thus prepared have been characterized using scanning electron microscopic (SEM), UV-vis spectroscopic, Fourier-transform-infrared (FTIR) spectroscopic and amperometric techniques, respectively. The results of photometric measurements carried out on tetraethylorthosilicate sol-gel/ChEt/ChOx reveal thermal stability up to 55 degrees C, response time as 180 s, linearity up to 780 mg dL(-1) (12 mM), shelf life of 1 month, detection limit of 12 mg dL(-1) and sensitivity as 5.4 x 10(-5) Abs. mg(-1) dL(-1).

Cite this paper

@article{Singh2007ImmobilizationOC, title={Immobilization of cholesterol esterase and cholesterol oxidase onto sol-gel films for application to cholesterol biosensor.}, author={Suman Singh and Rahul Singhal and Bansi Dhar Malhotra}, journal={Analytica chimica acta}, year={2007}, volume={582 2}, pages={335-43} }