Immobilization of cellulose fibrils on solid substrates for cellulase-binding studies through quantitative fluorescence microscopy.

@article{MoranMirabal2008ImmobilizationOC,
  title={Immobilization of cellulose fibrils on solid substrates for cellulase-binding studies through quantitative fluorescence microscopy.},
  author={Jos{\'e} Moran-Mirabal and Navaneetha Santhanam and St{\'e}phane C Corgi{\'e} and Harold G. Craighead and Larry P. Walker},
  journal={Biotechnology and bioengineering},
  year={2008},
  volume={101 6},
  pages={1129-41}
}
Cellulases, enzymes capable of depolymerizing cellulose polymers into fermentable sugars, are essential components in the production of bioethanol from lignocellulosic materials. Given the importance of these enzymes to the evolving biofuel industry considerable research effort is focused on understanding the interaction between cellulases and cellulose fibrils. This manuscript presents a method that addresses challenges that must be overcome in order to study such interactions through high… CONTINUE READING
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