Immobilization of cell-adhesive peptides to temperature-responsive surfaces facilitates both serum-free cell adhesion and noninvasive cell harvest.

@article{Ebara2004ImmobilizationOC,
  title={Immobilization of cell-adhesive peptides to temperature-responsive surfaces facilitates both serum-free cell adhesion and noninvasive cell harvest.},
  author={Mitsuhiro Ebara and Masayuki Yamato and T. Aoyagi and Akihiko Kikuchi and Kiyotaka Sakai and T. Okano},
  journal={Tissue engineering},
  year={2004},
  volume={10 7-8},
  pages={1125-35}
}
We have developed temperature-responsive cell culture surfaces to harvest intact cell sheets for tissue-engineering applications. Both cost and safety issues (e.g., prions, bovine spongiform encephalopathy) are compelling reasons to avoid use of animal-derived materials, including serum, in such culture. In the present study, synthetic cell-adhesive… CONTINUE READING