Immobilization of biomolecules onto silica and silica-based surfaces for use in planar array biosensors.

@article{ShriverLake2009ImmobilizationOB,
  title={Immobilization of biomolecules onto silica and silica-based surfaces for use in planar array biosensors.},
  author={Lisa C. Shriver-Lake and Paul T. Charles and Chris Rowe Taitt},
  journal={Methods in molecular biology},
  year={2009},
  volume={504},
  pages={419-40}
}
Several methods are described in which a biological recognition molecule--a critical element in any biosensor--is immobilized onto a silica or silica-based sensing substrate. Although several variations are described, the methods for covalent immobilization share a common theme and are generally composed of three steps: modification of the surface to add specific functional groups (using appropriate silanes or an amine or carboxyl-containing hydrogel), covalent attachment of a crosslinker… CONTINUE READING