Immobilization of Lipase from Pseudomonas fluorescens on Porous Polyurea and Its Application in Kinetic Resolution of Racemic 1-Phenylethanol.

@article{Han2016ImmobilizationOL,
  title={Immobilization of Lipase from Pseudomonas fluorescens on Porous Polyurea and Its Application in Kinetic Resolution of Racemic 1-Phenylethanol.},
  author={Hui Han and Yamei Zhou and Shusheng Li and Yinping Wang and Xiang Zheng Kong},
  journal={ACS applied materials & interfaces},
  year={2016},
  volume={8 39},
  pages={
          25714-25724
        }
}
A porous polyurea (PPU) was prepared through a simple protocol by reacting toluene diisocyanate with water in binary solvent of water-acetone. Its amine group was determined through spectrophotometric absorbance based on its iminization with p-nitrobenzaldehyde amines. PPU was then used as a novel polymer support for enzyme immobilization, through activation by glutaraldehyde followed by immobilization of an enzyme, lipase from Pseudomonas fluorescens (PFL), via covalent bonding with the amine… CONTINUE READING