Immobilization of Bacillus licheniformis α-amylase onto reactive polymer films.

@article{Cordeiro2011ImmobilizationOB,
  title={Immobilization of Bacillus licheniformis α-amylase onto reactive polymer films.},
  author={Ana L. Cordeiro and Tina Lenk and Carsten Werner},
  journal={Journal of biotechnology},
  year={2011},
  volume={154 4},
  pages={216-21}
}
Alpha-amylase was covalently immobilized onto maleic anhydride copolymer films preserving activity. The initial activity of the immobilized layers strongly depended on the immobilization solution, and on the physicochemical properties of the copolymer film. Higher enzyme loading (quantified by amino acid analysis using HPLC) and activity (measured by following starch hydrolysis) were attainable onto hydrophilic, highly swelling 3-D poly(ethylene-alt-maleic anhydride) (PEMA) copolymer films… CONTINUE READING