Immobilization of Antibodies on a Photoactive Self-Assembled Monolayer on Gold

  title={Immobilization of Antibodies on a Photoactive Self-Assembled Monolayer on Gold},
  author={Emmanuel Delamarche and Gajendran Sundarababu and Hans A. Biebuyck and Bruno Michel and Ch. Gerber and Hans Sigrist and Heiko Wolf and Helmut Prof Dr Ringsdorf and Nicolas Xanthopoulos and Hans J{\"o}rg Mathieu},
This paper presents a strategy for immobilizing biomolecules on a photoactivable surface. A self-assembled monolayer is prepared by adsorbing an ω-functionalized dialkyl disulfide on gold. Functional groups of this monolayer are converted in two steps into a benzophenone derivative with an overall yield of 50 ± 10%. Several independent techniques (ellipsometry, X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy, radiolabel assay, and autoradiography… 

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