Immobilization of β-Galactosidases from Lactobacillus on Chitin Using a Chitin-Binding Domain.

@article{Pham2017ImmobilizationO,
  title={Immobilization of β-Galactosidases from Lactobacillus on Chitin Using a Chitin-Binding Domain.},
  author={Mai-Lan Pham and Tatjana Leister and Hoang Anh Thu Nguyen and Bien-Cuong Do and Anh-Tuan Pham and Dietmar Haltrich and Montarop Yamabhai and Thu-Ha Nguyen and Tien-Thanh Nguyen},
  journal={Journal of agricultural and food chemistry},
  year={2017},
  volume={65 14},
  pages={
          2965-2976
        }
}
Two β-galactosidases from Lactobacillus, including a heterodimeric LacLM type enzyme from Lactobacillus reuteri L103 and a homodimeric LacZ type β-galactosidase from Lactobacillus bulgaricus DSM 20081, were studied for immobilization on chitin using a carbohydrate-binding domain (chitin-binding domain, ChBD) from a chitinolytic enzyme. Three recombinant enzymes, namely, LacLM-ChBD, ChBD-LacLM, and LacZ-ChBD, were constructed and successfully expressed in Lactobacillus plantarum WCFS1. Depending… CONTINUE READING
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