Immobilization and surface characterization of NeutrAvidin biotin-binding protein on different hydrogel interlayers.

@article{Vermette2003ImmobilizationAS,
  title={Immobilization and surface characterization of NeutrAvidin biotin-binding protein on different hydrogel interlayers.},
  author={Patrick Vermette and Thomas Gengenbach and Upulie Divisekera and Peter A Kambouris and H J Hans J Griesser and Laurence Meagher},
  journal={Journal of colloid and interface science},
  year={2003},
  volume={259 1},
  pages={13-26}
}
For a number of potential applications, it is desirable to immobilize avidin class molecules onto solid supports and exploit their ability to bind biotinylated molecules with high affinity. NeutrAvidin molecules were surface immobilized in various ways. In this study, NeutrAvidin was covalently attached by carbodiimide chemistry onto carboxyl groups of polyacrylic acid and carboxymethyl-dextran hydrogel interlayers. A third strategy involved the affinity "docking" of NeutrAvidin onto a… CONTINUE READING

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