Immobilization and stretching of 5'-pyrene-terminated DNA on carbon film deposited on electron microscope grid.

The immobilization and stretching of randomly coiled DNA molecules on hydrophobic carbon film is a challenging microscopic technique, which possess various applications, especially for genome sequencing. In this report the pyrenyl nucleus is used as an anchor moiety to acquire higher affinity of double stranded DNA to the graphite surface. DNA and pyrene… CONTINUE READING