Immobilization and stabilization of invertase using specific polyclonal antibodies.

@article{Jafri1993ImmobilizationAS,
  title={Immobilization and stabilization of invertase using specific polyclonal antibodies.},
  author={F Jafri and Saba Husain and Mohammed Saleemuddin},
  journal={Biotechnology and applied biochemistry},
  year={1993},
  volume={18 ( Pt 3)},
  pages={401-8}
}
Antisera raised in rabbits to baker's-yeast invertase significantly activated the enzyme in vitro. The antisera contained precipitating antibodies, a significant fraction of which appeared to be directed against the glycosyl residues of the enzyme. Invertase could be immobilized as insoluble enzyme antibody adducts or by binding to a Sepharose matrix precoupled with the gamma-globulin fraction derived from the antisera. The immobilized invertase preparations exhibited high enzyme activity and… CONTINUE READING