Immobilization and stabilization of horseradish peroxidase isoforms.

  title={Immobilization and stabilization of horseradish peroxidase isoforms.},
  author={Saba Husain and F Jafri and Mohammed Saleemuddin},
  journal={Biochemistry and molecular biology international},
  volume={40 1},
Purified anionic and cationic isoforms of horseradish peroxidase (HRP) immobilized by coupling the amino acid side-chain amino groups and/or carbohydrate moieties to Sepharose have been studied for their resistance to denaturation. The isoforms were treated with periodate followed by ethylenediamine to generate additional amino groups in the glycosyl residues. The immobilized preparations were: Preparation I (Sp-aHRP, Sp-cHRP), in which HRP was covalently immobilized via side-chain amino groups… CONTINUE READING