Immobilization and activity assay of cytochrome P450 on patterned lipid membranes.

@article{Ueda2007ImmobilizationAA,
  title={Immobilization and activity assay of cytochrome P450 on patterned lipid membranes.},
  author={Yoshihiro Ueda and Kenichi Morigaki and Yoshiro Tatsu and Noboru Yumoto and Hiromasa Imaishi},
  journal={Biochemical and biophysical research communications},
  year={2007},
  volume={355 4},
  pages={926-31}
}
We report on a methodology for immobilizing cytochrome P450 on the surface of micropatterned lipid bilayer membranes and measuring the enzymatic activity. The patterned bilayer comprised a matrix of polymeric lipid bilayers and embedded fluid lipid bilayers. The polymeric lipid bilayer domains act as a barrier to confine fluid lipid bilayers in defined… CONTINUE READING