Immobilization and Kinetics of Catalase on Calcium Carbonate Nanoparticles Attached Epoxy Support

  title={Immobilization and Kinetics of Catalase on Calcium Carbonate Nanoparticles Attached Epoxy Support},
  author={Preety and Vinita Hooda},
  journal={Applied Biochemistry and Biotechnology},
  • Preety, Vinita Hooda
  • Published 2013
  • Chemistry, Medicine
  • Applied Biochemistry and Biotechnology
  • A novel hybrid epoxy/nano CaCO3 composite matrix for catalase immobilization was prepared by polymerizing epoxy resin in the presence of CaCO3 nanoparticles. The hybrid support was characterized using scanning electron microscopy and Fourier transform infrared spectroscopy. Catalase was successfully immobilized onto epoxy/nano CaCO3 support with a conjugation yield of 0.67 ± 0.01 mg/cm2 and 92.63 ± 0.80 % retention of activity. Optimum pH and optimum temperature of free and immobilized… CONTINUE READING
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