Immobilisation of living bacteria for AFM imaging under physiological conditions.

@article{Meyer2010ImmobilisationOL,
  title={Immobilisation of living bacteria for AFM imaging under physiological conditions.},
  author={Rikke Louise Meyer and Xingfei Zhou and Lone Tang and Ayyoob Arpanaei and Peter Kingshott and Flemming Besenbacher},
  journal={Ultramicroscopy},
  year={2010},
  volume={110 11},
  pages={
          1349-57
        }
}
Atomic force microscopy (AFM) holds great potential for studying the nanoscale surface structures of living cells, and to measure their interactions with abiotic surfaces, other cells, or specific biomolecules. However, the application of AFM in microbiology is challenging due to the difficulty of immobilising bacterial cells to a flat surface without changing the cell surface properties or cell viability. We have performed an extensive and thorough study of how to functionalise surfaces in… CONTINUE READING
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