Immobilisation of DNA to polymerised SU-8 photoresist.

@article{Marie2006ImmobilisationOD,
  title={Immobilisation of DNA to polymerised SU-8 photoresist.},
  author={Rodolphe Marie and Silvan Schmid and Alicia Johansson and Louise Ejsing and Maria Nordstr{\"o}m and Daniel H{\"a}fliger and Claus Bv Christensen and Anja Boisen and Martin Dufva},
  journal={Biosensors & bioelectronics},
  year={2006},
  volume={21 7},
  pages={1327-32}
}
SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalize SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm(2) as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture… CONTINUE READING

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