Immobilisation and mechanical, support of individual protoplasts.

  title={Immobilisation and mechanical, support of individual protoplasts.},
  author={Peter Scheurich and Heide Schnabl and U. Zimmermann and Jochen Klein},
  journal={Biochimica et biophysica acta},
  volume={598 3},
Mesophyl cell protoplasts of Vicia faba were suspended in a solution consisting of 10% sodium alginate and 0.4 M mannitol. The protoplasts could be immobilized by cross-linking the alginate in the presence of 100 mM CaCl2. Changes in the osmolarity of the external medium led to reversible shrinkage and swelling of the entrapped protoplasts. It was demonstrated by using the pressure probe technique that a pressure gradient (cell turgor pressure) of several 100 mbar is built up when the… CONTINUE READING

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