Immature transformed rat islet beta-cells differentially express C-peptides derived from the genes coding for insulin I and II as well as a transfected human insulin gene.

@article{Blume1992ImmatureTR,
  title={Immature transformed rat islet beta-cells differentially express C-peptides derived from the genes coding for insulin I and II as well as a transfected human insulin gene.},
  author={Niels G{\"o}ran Blume and Jacob Sten Petersen and L C Andersen and Hans Kofod and Thomas Dyrberg and Birgitte Koch Michelsen and Palle Serup and Ole Dragsbaek Madsen},
  journal={Molecular endocrinology},
  year={1992},
  volume={6 2},
  pages={299-307}
}
Synthetic peptides representing unique sequences in rat proinsulin C-peptide I and II were used to generate highly specific antisera, which, when applied on sections of normal rat pancreas, confirm a homogeneous coexpression of the two C-peptides in all islet beta-cells. Insulin gene expression is induced in the transformed heterogeneous rat islet cell clone, NHI-6F, by transient in vivo passage. During this process a transfected human insulin gene is coactivated with the endogenous nonallelic… CONTINUE READING

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