Imaging yeast NPCs: from classical electron microscopy to Immuno-SEM.

@article{Kiseleva2014ImagingYN,
  title={Imaging yeast NPCs: from classical electron microscopy to Immuno-SEM.},
  author={Elena V. Kiseleva and A. Christine Richardson and Jindři{\vs}ka Fi{\vs}erov{\'a} and Anton Strunov and Matthew C. Spink and Simeon R Johnson and Martin W. Goldberg},
  journal={Methods in cell biology},
  year={2014},
  volume={122},
  pages={59-79}
}
Electron microscopy (EM) has been used extensively for the study of nuclear transport as well as the structure of the nuclear pore complex (NPC) and nuclear envelope. However, there are specific challenges faced when carrying out EM in one of the main model organisms used: the yeast, Saccharomyces cerevisiae. These are due to the presence of a cell wall… CONTINUE READING