Imaging the cell entry of the anthrax oedema and lethal toxins with fluorescent protein chimeras.

@article{Zornetta2010ImagingTC,
  title={Imaging the cell entry of the anthrax oedema and lethal toxins with fluorescent protein chimeras.},
  author={Irene Zornetta and Lucia Brandi and Blythe E. Janowiak and Federica Dal Molin and Fiorella Tonello and Robert J. Collier and Cesare Montecucco},
  journal={Cellular microbiology},
  year={2010},
  volume={12 10},
  pages={1435-45}
}
To investigate the cell entry and intracellular trafficking of anthrax oedema factor (EF) and lethal factor (LF), they were C-terminally fused to the enhanced green fluorescent protein (EGFP) and monomeric Cherry (mCherry) fluorescent proteins. Both chimeras bound to the surface of BHK cells treated with protective antigen (PA) in a patchy mode. Binding was followed by rapid internalization, and the two anthrax factors were found to traffic along the same endocytic route and with identical… CONTINUE READING