Imaging the alphavirus exit pathway.

@article{Martnez2014ImagingTA,
  title={Imaging the alphavirus exit pathway.},
  author={Mar{\'i}a Guadalupe Calder{\'o}n Mart{\'i}nez and Erik-Lee Snapp and Geoffrey S. Perumal and Frank P. Macaluso and Margaret Kielian},
  journal={Journal of virology},
  year={2014},
  volume={88 12},
  pages={6922-33}
}
UNLABELLED Alphaviruses are small enveloped RNA viruses with highly organized structures that exclude host cell proteins. They contain an internal nucleocapsid and an external lattice of the viral E2 and E1 transmembrane proteins. Alphaviruses bud from the plasma membrane (PM), but the process and dynamics of alphavirus assembly and budding are poorly understood. Here we generated Sindbis viruses (SINVs) with fluorescent protein labels on the E2 envelope protein and exploited them to… CONTINUE READING
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