Imaging signal transduction in living cells with GFP-based probes.

@article{Zaccolo2000ImagingST,
  title={Imaging signal transduction in living cells with GFP-based probes.},
  author={Manuela Zaccolo and Tullio Pozzan},
  journal={IUBMB life},
  year={2000},
  volume={49 5},
  pages={375-9}
}
Since the cloning and the eterologous expression of the Green Fluorescence Protein (GFP), a number of applications have been reported where protein location within the cell or gene expression is revealed by fluorescent imaging of living cells. Modified GFPs, however, can now be exploited not only as a fluorescent reporter but also as a dynamic marker of… CONTINUE READING