Imaging real-time proteolysis of single collagen I molecules with an atomic force microscope.

@article{Lin1999ImagingRP,
  title={Imaging real-time proteolysis of single collagen I molecules with an atomic force microscope.},
  author={Hai Lin and Dennis O Clegg and R. Jawahar Lal},
  journal={Biochemistry},
  year={1999},
  volume={38 31},
  pages={9956-63}
}
The dynamic process of synthesis and degradation of extracellular matrix molecules, including various collagens, is important in normal physiological functions and pathological conditions. Existing models of collagen enzymatic degradation reactions are derived from bulk biochemical assays. In this study, we have imaged in real-time individual collagen I molecules and their proteolysis by Clostridium histolyticum collagenases in phosphate-buffered saline (PBS) with atomic force microscopy (AFM… CONTINUE READING
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