Imaging of protein cluster sizes by means of confocal time-gated fluorescence anisotropy microscopy.

@article{Bader2007ImagingOP,
  title={Imaging of protein cluster sizes by means of confocal time-gated fluorescence anisotropy microscopy.},
  author={Arjen N. Bader and Erik G Hofman and Paul M P van Bergen en Henegouwen and Hans C. Gerritsen},
  journal={Optics express},
  year={2007},
  volume={15 11},
  pages={6934-45}
}
A time-resolved fluorescence anisotropy imaging method for studying nanoscale clustering of proteins or lipids was developed and evaluated. It is based on FRET between the identical fluorophores (homo-FRET), which results in a rapid depolarization of the fluorescence. The method employs the time-resolved fluorescence anisotropy decays recorded in a confocal microscope equipped with pulsed excitation and time-gated detection. From the decay the limiting anisotropy r(inf) was derived, which is a… CONTINUE READING

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