Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography☆

  title={Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography☆},
  author={Osnath Assayag and Kate Grieve and Bertrand Devaux and Fabrice Harms and Johan Pallud and Fabrice Chr{\'e}tien and Claude A. Boccara and Pascale Varlet},
  journal={NeuroImage : Clinical},
  pages={549 - 557}

Figures and Tables from this paper

Spectral domain optical coherence tomography for ex vivo brain tumor analysis

In both, histopathological diagnosis and the analysis of the OCT images, different types of brain tumor showed difference in structure, and the difference between two images of samples taken directly after surgery is less distinct.

Feasibility evaluation of micro-optical coherence tomography (μOCT) for rapid brain tumor type and grade discriminations: μOCT images versus pathology

Micro-optical coherence tomography imaging results could help discriminate both tumor types and grades, which illustrates the potential of μOCT as an intraoperative diagnostic imaging tool to help neurosurgeons perform their surgery precisely in tumor treatment process.

Study of malignant brain gliomas using optical coherence tomography and terahertz pulsed spectroscopy aimed on advanced intraoperative neurodiagnosis

Ex vivo study of malignant brain gliomas featuring different grades by means of optical coherence tomography and terahertz pulsed spectroscopy shows potential of TPS to differentiate intact and malignant tissues and the potential of OCT to differentiate low- and high-grade glioma as well as intact tissue and low-gradegliomas.

Spectroscopic optical coherence tomography for ex vivo brain tumor analysis

Spectroscopic Optical Coherence Tomography was applied to further enhance the differences between the tissue types and pattern recognition and machine learning algorithms were applied to classify the derived spectroscopic information.

Differentiation of healthy and malignant brain tissues using terahertz pulsed spectroscopy and optical coherence tomography

This research studied the ability of dual-modality imaging that combines such methods as optical coherence tomography and terahertz pulsed spectroscopy, for intraoperative diagnosis of brain tumors with a strong emphasize on a human brain gliomas to highlight a potential of the considered instruments in the label-free intraoperative neurodiagnostics.

Improved Diagnostic Imaging of Brain Tumors by Multimodal Microscopy and Deep Learning

A hybrid prototype combining visible light optical coherence microscopy (OCM) and high-resolution fluorescence imaging for assessment of brain tumor samples acquired by 5-aminolevulinic acid (5-ALA) fluorescence-guided surgery offers potential to translate into an in vivo imaging setup for substantially improved intraoperative guidance of brain tumors surgeries.

Automated differentiation between meningioma and healthy brain tissue based on optical coherence tomography ex vivo images using texture features

A preliminary study toward in vivo brain tumor removal assistance by investigating meningioma, healthy white, and healthy gray matter by using a commercially available OCT device and employing different algorithms to extract texture features and apply pattern recognition methods for their classification.

Optical coherence tomography of human brain glioma as a promising tool for intraoperative diagnostics in neurosurgery

This research, aimed at the study of the ability of OCT for the intraoperative diagnosis of brain gliomas of different grades, has the goal to observe the differences between OCT signals obtained for ex vivo samples of various types of human brainglioma and intact brain tissue.

Pilot feasibility study of in vivo intraoperative quantitative optical coherence tomography of human brain tissue during glioma resection

It is concluded that the proposed method for quantitative in vivo OCT of human brain tissue is feasible during glioma resection surgery and in the range of previously reported values.

High-Speed Three-Dimensional Glioma Morphology Imaging and Grade Discrimination using Micro-Optical Coherence Tomography

A free-spaced micro-optical coherence tomography system is constructed, which achieves a spatial resolution of ~ 2.0 μm, and its capability for identifying the cellular/sub-cellular structures of glioma lesions is evaluated, demonstrating the potential of μOCT for neurosurgery in clinical practice.



Imaging of human brain tumor tissue by near-infrared laser coherence tomography

This feasibility study has demonstrated that OCT analysis of the tissue microstructure and light attenuation characteristics discriminate normal brain, areas of tumor infiltrated brain, solid tumor, and necrosis.

Optical coherence tomography for neurosurgical imaging of human intracortical melanoma.

OCT can effectively differentiate normal cortex from intracortical melanoma based on variations in optical backscatter, and may permit the intraoperative identification of tumor and the more precise localization of tumor margins.

Imaging ex vivo healthy and pathological human brain tissue with ultra-high-resolution optical coherence tomography.

The ability of UHR OCT to visualize and identify morphological features such as microcalcifications, enlarged nuclei of tumor cells, small cysts, and blood vessels, which are characteristic of neuropathologies and normally absent in healthy brain tissue are demonstrated.

Large Field, High Resolution Full-Field Optical Coherence Tomography

High-resolution Full-Field Optical Coherence Tomography (FF-OCT) was used to image human breast tissue and is evaluated to assess its ability to aid the pathologist's management of intra-operative diagnoses.

Intraoperative Confocal Microscopy for Brain Tumors: A Feasibility Analysis in Humans

Preliminary analysis demonstrates reliability for a variety of lesions in identifying tumor cells and the tumor-brain interface in intraoperative confocal microscopy for brain tumor resection.

Identifying brain neoplasms using dye-enhanced multimodal confocal imaging.

Multimodal confocal imaging has the potential to aid in the intraoperative detection of microscopic deposits of brain neoplasms and the application of this technique may improve completeness of resection and increase patient survival.

Benign and malignant lesions in the human breast depicted with ultrahigh resolution and three-dimensional optical coherence tomography.

Imaging of ductal carcinoma in situ, infiltrating cancer, and microcalcifications correlated with corresponding histopathologic findings showed OCT is potentially useful for visualization of breast lesions at a resolution greater than that of currently available clinical imaging methods.

Intraoperative evaluation of breast tumor margins with optical coherence tomography.

Results show the potential of OCT as a real-time method for intraoperative margin assessment in breast-conserving surgeries and areas of higher scattering tissue with a heterogeneous pattern were indicative of tumor cells and tumor tissue in contrast to lower scattering adipocytes found in normal breast tissue.

Multimodal full-field optical coherence tomography on biological tissue: toward all optical digital pathology

A multimodal approach to FF-OCT is reported, combining two Full-Field techniques for collecting a backscattered endogeneous OCT image and a fluorescence exogeneous image in parallel, and paves the way for improvements of standard pathology procedures, as a faster, non sacrificial, operator independent digital optical method compared to frozen sections.

Integrated optical coherence tomography and microscopy for ex vivo multiscale evaluation of human breast tissues.

The results lay the foundation for future in vivo optical evaluation of breast tissues, using OCT and OCM, which has the potential to guide core needle biopsies, assess surgical margins, and evaluate nodal involvement in breast cancer.