Imaging of muscarinic acetylcholine receptor signaling in hippocampal neurons: evidence for phosphorylation-dependent and -independent regulation by G-protein-coupled receptor kinases.

@article{Willets2004ImagingOM,
  title={Imaging of muscarinic acetylcholine receptor signaling in hippocampal neurons: evidence for phosphorylation-dependent and -independent regulation by G-protein-coupled receptor kinases.},
  author={J M Willets and Mark S. Nash and R. A. John Challiss and Stefan R. Nahorski},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={2004},
  volume={24 17},
  pages={4157-62}
}
We used the inositol 1,4,5-trisphosphate (IP3) biosensor, the pleckstrin homology (PH) domain of PLCdelta1 (phospholipase C) tagged with enhanced green fluorescent protein (eGFP-PH(PLCdelta)), to examine muscarinic acetylcholine (mACh) receptor regulation of phospholipase C/IP3 signaling in intact single hippocampal neurons in "real time." Initial experiments produced a pharmacological profile consistent with the presence of a predominant M1 mACh receptor population coupled to the IP3 response… CONTINUE READING

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Wedegaertner PB (2003) G proteincoupled receptor kinase 2/G q/11 interaction

  • R Sterne-Marr, JJ Tesmer, +5 authors JL Benovic
  • J Biol Chem
  • 2003
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