Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes.

@article{Qu2008ImagingOC,
  title={Imaging of cancer cells by multiphoton microscopy using gold nanoparticles and fluorescent dyes.},
  author={Xiaochao Qu and Jing Wang and Zhenxi Zhang and Norbert Koop and Ramtin Rahmanzadeh and Gereon Huettmann},
  journal={Journal of biomedical optics},
  year={2008},
  volume={13 3},
  pages={031217}
}
Due to their unique optical properties, optical probes, including metal nanoparticles (NPs) and fluorescent dyes, are increasingly used as labeling tools in biological imaging. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) at 750-nm excitation, we recorded intensity and FLIM images from gold NPs (30 nm) and the fluorescent dye Alexa 488 (A488) conjugated with monoclonal ACT-1 antibodies as well as Hoechst 33258 (H258) after incubation with the lymphoma cell line (Karpas… CONTINUE READING
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