Imaging of bacterial multicellular behaviour in biofilms in liquid by atmospheric scanning electron microscopy.

@article{Sugimoto2016ImagingOB,
  title={Imaging of bacterial multicellular behaviour in biofilms in liquid by atmospheric scanning electron microscopy.},
  author={Shinya Sugimoto and Ken-ichi Okuda and Reina Miyakawa and Mari Sato and Ken-ichi Arita-Morioka and Akio Chiba and Kunitoshi Yamanaka and Teru Ogura and Yoshimitsu Mizunoe and Chikara Sato},
  journal={Scientific reports},
  year={2016},
  volume={6},
  pages={25889}
}
Biofilms are complex communities of microbes that attach to biotic or abiotic surfaces causing chronic infectious diseases. Within a biofilm, microbes are embedded in a self-produced soft extracellular matrix (ECM), which protects them from the host immune system and antibiotics. The nanoscale visualisation of delicate biofilms in liquid is challenging. Here, we develop atmospheric scanning electron microscopy (ASEM) to visualise Gram-positive and -negative bacterial biofilms immersed in… CONTINUE READING

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