Imaging of Microglia Activation in Stroke

@article{Thiel2011ImagingOM,
  title={Imaging of Microglia Activation in Stroke},
  author={Alexander Thiel and W.-D. Heiss},
  journal={Stroke},
  year={2011},
  volume={42},
  pages={507-512}
}
Activated microglia is one of the most important cellular components of poststroke neuroinflammation, which occurs early in the area of the infarct but also in remote regions with fiber tract connections to the site of the primary lesion. The development of different radioligands for the translocator protein, a mitochondrial membrane protein expressed in microglial cells when they transform from the resting to the activated state, allows to study the temporal dynamics of this cellular… 

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References

SHOWING 1-10 OF 46 REFERENCES

In vivo imaging of activated microglia using [11 C]PK11195 and positron emission tomography in patients after ischemic stroke

This PET method is considered as a promising tool to study the late pathogenetic consequences of cerebral infarction and to evaluate neuroprotective strategies with respect to the consequences of the microglial activation.

Imaging Microglial Activation During Neuroinflammation and Alzheimer’s Disease

Imaging microglia in vivo in live subjects by confocal or two/multiphoton microscopy offers the advantage of studying these cells over time in their native environment, and can offer a measure of the inflammatory process and a means of detecting progression of disease and efficacy of therapeutics over time.

Intrinsic Activated Microglia Map to the Peri-infarct Zone in the Subacute Phase of Ischemic Stroke

In ischemic stroke patients, minimal activation of microglia is seen before 72 hours and extending to 30 days in core infarction, contralateral hemisphere, and peri-infarct zone, suggesting a therapeutic opportunity that extends beyond time windows traditionally reserved for neuroprotection.

Imaging Inflammation in Acute Brain Ischemia

Iron oxide nanoparticles such as ultrasmall superparamagnetic iron oxide (USPIO) are novel cell-specific contrast agents for MRI, and USPIO-laden macrophages cause typical signal changes in MRI of infarcted brain parenchyma, which has been demonstrated in studies of both experimental ischemia and human stroke.

Microglia in ischemic brain injury.

Microglia are resident CNS immune cells that are active sensors in healthy brain and versatile effectors under pathological conditions. Cerebral ischemia induces a robust neuroinflammatory response

In vivo imaging of neuroinflammation

Proliferating Resident Microglia after Focal Cerebral Ischaemia in Mice

  • Á. DénesR. Vidyasagar S. Allan
  • Biology
    Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
  • 2007
Interestingly, the more severe injury associated with 60 mins of MCAo leads to a markedly reduced proliferation of resident microglial cells, suggesting that these cells may play a protective function, possibly through phagocytosis of infiltrating neutrophils, which further support possible beneficial actions of microglia cells in the injured brain.

Microglia: active sensor and versatile effector cells in the normal and pathologic brain

This review focuses on several key observations that illustrate the multi-faceted activities of microglia in the normal and pathologic brain.

The Temporal Dynamics of Poststroke Neuroinflammation: A Longitudinal Diffusion Tensor Imaging–Guided PET Study with 11C-PK11195 in Acute Subcortical Stroke

DTI-guided 11C-PK11195 PET in acute subcortical stroke demonstrates differential temporal dynamics of local and remote AMG, which might contribute differently to clinical outcome.