Imaging of Borrelia turicatae Producing the Green Fluorescent Protein Reveals Persistent Colonization of the Ornithodoros turicata Midgut and Salivary Glands from Nymphal Acquisition through Transmission.

@article{Krishnavajhala2017ImagingOB,
  title={Imaging of Borrelia turicatae Producing the Green Fluorescent Protein Reveals Persistent Colonization of the Ornithodoros turicata Midgut and Salivary Glands from Nymphal Acquisition through Transmission.},
  author={Aparna Krishnavajhala and Hannah K. Wilder and William K. Boyle and Ashish Damania and Justin A. Thornton and Adalberto Angel P{\'e}rez de Le{\'o}n and Pete D. Teel and Job E Lopez},
  journal={Applied and environmental microbiology},
  year={2017},
  volume={83 5}
}
Relapsing fever (RF) spirochetes colonize and are transmitted to mammals primarily by Ornithodoros ticks, and little is known regarding the pathogen's life cycle in the vector. To further understand vector colonization and transmission of RF spirochetes, Borrelia turicatae expressing a green fluorescent protein (GFP) marker (B. turicatae-gfp) was generated. The transformants were evaluated during the tick-mammal infectious cycle, from the third nymphal instar to adult stage. B. turicatae-gfp… CONTINUE READING
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