Imaging neuronal activity during zebrafish behavior with a genetically encoded calcium indicator.

@article{Higashijima2003ImagingNA,
  title={Imaging neuronal activity during zebrafish behavior with a genetically encoded calcium indicator.},
  author={S. Higashijima and M. A. Masino and G. Mandel and J. Fetcho},
  journal={Journal of neurophysiology},
  year={2003},
  volume={90 6},
  pages={
          3986-97
        }
}
Genetically encoded calcium indicators, such as cameleon, have offered the promise of noninvasively monitoring activity of neurons, but no one has demonstrated whether these indicators can report calcium transients in neurons of behaving vertebrates. We show that cameleon can be expressed at high levels in sensory and spinal cord neurons in zebrafish by using neural-specific promoters in both transient expression experiments and in a stable transgenic line. Using standard confocal microscopy… Expand
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