Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein.

@article{Akemann2012ImagingNC,
  title={Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein.},
  author={W. Akemann and H. Mutoh and A. Perron and Yun Kyung Park and Y. Iwamoto and T. Kn{\"o}pfel},
  journal={Journal of neurophysiology},
  year={2012},
  volume={108 8},
  pages={
          2323-37
        }
}
Population signals from neuronal ensembles in cortex during behavior are commonly measured with EEG, local field potential (LFP), and voltage-sensitive dyes. A genetically encoded voltage indicator would be useful for detection of such signals in specific cell types. Here we describe how this goal can be achieved with Butterfly, a voltage-sensitive fluorescent protein (VSFP) with a subthreshold detection range and enhancements designed for voltage imaging from single neurons to brain in vivo… Expand

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References

SHOWING 1-10 OF 70 REFERENCES
Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins
TLDR
It is shown that genetically encoded voltage-sensitive fluorescent proteins (VSFPs) provide an optical voltage report from targeted neurons in culture, acute brain slices and living mice, and that these probes can report cortical electrical responses to single sensory stimuli in vivo. Expand
Red-shifted voltage-sensitive fluorescent proteins.
TLDR
A series of genetically encoded voltage-sensitive fluorescent proteins are generated by molecular fusion of the voltage-sensing domain of Ci-VSP (Ciona intestinalis voltage sensor-containing phosphatase) to red-shifted fluorescent protein operands and shown how these indicator proteins convert voltage-dependent structural rearrangements into a modulation of fluorescence output. Expand
Combined voltage and calcium epifluorescence imaging in vitro and in vivo reveals subthreshold and suprathreshold dynamics of mouse barrel cortex.
TLDR
Combining VSD and CaSD measurements can be used to specify the temporal and spatial relationships between subthreshold and suprathreshold activity of the neocortex. Expand
Effect of voltage sensitive fluorescent proteins on neuronal excitability.
TLDR
Using computational neuron simulations, quantitatively investigated the perturbing effects of sensing capacitance on the input/output relationship in two central neuron models, a cerebellar Purkinje and a layer 5 pyramidal neuron and suggested ways to reduce capacitive effects at a given level of signal/noise. Expand
Long-term voltage-sensitive dye imaging reveals cortical dynamics in behaving monkeys.
TLDR
It is found that VSDI emphasizes subthreshold activity more than imaging based on intrinsic signals, that emphasized more spiking activity, on comparing the relative amplitudes of the evoked signals and differential map obtained using these two different imaging methodologies. Expand
A Genetically Encoded Optical Probe of Membrane Voltage
TLDR
A novel, genetically encoded probe is constructed that can be used to measure transmembrane voltage in single cells by fused a modified green fluorescent protein into a voltage-sensitive K+ channel so that voltage-dependent rearrangements in the K- channel would induce changes in the fluorescence of GFP. Expand
Genetically encoded probes for optical imaging of brain electrical activity.
TLDR
The conceptual motivation for optogenetic recording of brain electrical activity using genetically encoded voltage-sensitive fluorescent proteins (VSFPs) is laid out, how the current generation of VSFPs has evolved is described, and how VS FPs report membrane voltage signals in isolated cells, brain slices, and living animals are demonstrated. Expand
Millisecond-timescale, genetically targeted optical control of neural activity
Temporally precise, noninvasive control of activity in well-defined neuronal populations is a long-sought goal of systems neuroscience. We adapted for this purpose the naturally occurring algalExpand
Optical probing of neuronal circuit dynamics: genetically encoded versus classical fluorescent sensors
TLDR
This work critically review the development of these new probes, and analyzes objectives and experimental conditions in which classical probes are likely to prevail and where the fluorescent protein sensors will open paths to previously unexplored territories of functional neuroimaging. Expand
Real-time optical imaging of naturally evoked electrical activity in intact frog brain
TLDR
It is demonstrated that optical measurements can be used for real-time imaging of spatio-temporal patterns of neuronal responses and for identification of functional units evoked by natural visual stimuli. Expand
...
1
2
3
4
5
...