Imaging live cells grown on a three dimensional collagen matrix using Raman microspectroscopy.

@article{Bonnier2010ImagingLC,
  title={Imaging live cells grown on a three dimensional collagen matrix using Raman microspectroscopy.},
  author={Franck Bonnier and P. Knief and Bora Lim and Aidan D. Meade and Jennifer Dorney and Kunal Bhattacharya and Fiona M. Lyng and Hugh J. Byrne},
  journal={The Analyst},
  year={2010},
  volume={135 12},
  pages={3169-77}
}
Three dimensional collagen gels have been used as matrices for the imaging of live cells by Raman spectroscopy. The study is conducted on a human lung adenocarcinoma (A549) and a spontaneously immortalized human epithelial keratinocyte (HaCaT) cell line. The lateral resolution of the system has been estimated to be <1.5 μm making it possible to access the subcellular organization. Using K-means clustering analysis, it is shown that the different subcellular compartments of individual cells can… CONTINUE READING