Imaging individual mRNA molecules using multiple singly labeled probes

@inproceedings{Raj2008ImagingIM,
  title={Imaging individual mRNA molecules using multiple singly labeled probes},
  author={A. Clement Louis Raj and Patrick T C van den Bogaard and Scott A. Rifkin and Alexander van Oudenaarden and Sanjay Tyagi},
  booktitle={Nature Methods},
  year={2008}
}
We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot by fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons. 
BETA

Figures and Topics from this paper.

Explore Further: Topics Discussed in This Paper

Citations

Publications citing this paper.
SHOWING 1-10 OF 675 CITATIONS, ESTIMATED 54% COVERAGE

Distribution shapes govern the discovery of predictive models for gene regulation

  • Proceedings of the National Academy of Sciences of the United States of America
  • 2018
VIEW 8 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

RNA Detection

  • Methods in Molecular Biology
  • 2018
VIEW 8 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

2009
2019

CITATION STATISTICS

  • 93 Highly Influenced Citations

  • Averaged 77 Citations per year over the last 3 years

References

Publications referenced by this paper.

Gut a b c A P en dpp d e Merge of focal planes Single focal plane

M. Sanicola, J. Sekelsky, S. Elson, W. M. Gelbart
  • Genetics 139,
  • 1995

Similar Papers

Loading similar papers…