Imaging Switchable Protein Interactions with an Active Porous Polymer Support.

@article{Dutta2020ImagingSP,
  title={Imaging Switchable Protein Interactions with an Active Porous Polymer Support.},
  author={Chayan Dutta and Logan D. C. Bishop and Jorge Zepeda O and Sudeshna Chatterjee and Charlotte Flatebo and Christy F. Landes},
  journal={The journal of physical chemistry. B},
  year={2020}
}
Mechanistic details about how local physicochemistry of porous interfaces drives protein transport mechanisms are necessary to optimize biomaterial applications. Cross-linked hydrogels made of stimuli-responsive polymers have potential for active protein capture and release through tunable steric and chemical transformations. Simultaneous monitoring of dynamic changes in both protein transport and interfacial polymer structure is an experimental challenge. We use single-particle tracking (SPT… 
The competing influence of surface roughness, hydrophobicity, and electrostatics on protein dynamics on a self-assembled monolayer.
Surface morphology, in addition to hydrophobic and electrostatic effects, can alter how proteins interact with solid surfaces. Understanding the heterogeneous dynamics of protein adsorption on
Super-resolution fluorescence imaging of extracellular environments.
Computationally-efficient spatiotemporal correlation analysis super-resolves anomalous diffusion
TLDR
The least-squares curve fitting step is parallelized on a GPU improving computation times by up to a factor of 40, anomalous diffusion and two-component Brownian diffusion models are implemented, and fcsSOFI is made more accessible by packaging it in a user-friendly GUI.
Computationally-efficient spatiotemporal correlation analysis super-resolves anomalous diffusion.
TLDR
The least-squares curve fitting step is parallelized on a GPU improving computation times by up to a factor of 40, anomalous diffusion and two-component Brownian diffusion models are implemented, and fcsSOFI is made more accessible by packaging it in a user-friendly GUI.
Transforming Separation Science with Single-Molecule Methods.
TLDR
This work outlines single-molecule fluorescence techniques that can identify key interactions under ambient conditions and describes how studying increasingly complex samples heighten the relevance of single-Molecule results to industrial applications.
Untying the Gordian KNOT: Unbiased Single Particle Tracking Using Point Clouds and Adaptive Motion Analysis.
TLDR
This work presents an unbiased single particle tracking algorithm: Knowing Nothing Outside Tracking (KNOT), which uses point clouds provided by iterative deconvolution to educate individual particle localizations and link particle positions between frames to achieve 2-D and 3-D tracking.
Time-Resolved Microscopy: A New Frontier in Physical Chemistry.

References

SHOWING 1-10 OF 49 REFERENCES
Direct Imaging of Protein Stability and Folding Kinetics in Hydrogels.
TLDR
The imaging capabilities of FReI, demonstrated to be diffraction limited, further revealed spatially homogeneous protein unfolding across the hydrogels at 500 nm length scales and revealed differences in protein properties at the gel-solution boundary.
Fluorescence microscopy of biophysical protein dynamics in nanoporous hydrogels
TLDR
In situ observations of proteins in hydrogels using fluorescence microscopies can inform and inspire soft nanomaterial design to improve the performance, shelf life, and cost of biomaterials.
Stimuli‐Responsive Porous Hydrogels at Interfaces for Molecular Filtration, Separation, Controlled Release, and Gating in Capsules and Membranes
TLDR
A continuously growing area of controlled and tunable transport and separation of biomolecules and drugs has recently attracted attention to the structures, referred to as stimuli‐responsive porous hydrogel thin films, which have a low thickness with well‐defined size and shape of pores.
Variable Lysozyme Transport Dynamics on Oxidatively Functionalized Polystyrene Films.
TLDR
Single-molecule fluorescence microscopy is utilized to determine the adsorption dynamics of lysozyme at the interface of polystyrene oxidized via UV exposure and oxygen plasma and functionalized by ligand grafting to produce varying degrees of surface hydrophilicity, surface roughness, and induced oxygen content.
Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging.
TLDR
A super-resolution optical image is produced that simultaneously characterizes the nanometer dimensions of and diffusion dynamics within porous structures by correlating stochastic fluctuations from diffusing fluorescent probes in the pores of the sample, dubbed here as "fluorescence correlation spectroscopy super- resolution optical fluctuation imaging" or "fcsSOFI".
Stimulus-responsive hydrogels: Theory, modern advances, and applications.
Transport in supported polyelectrolyte brushes.
TLDR
Recent progress is discussed in using fluorescence correlation spectroscopy, single-molecule polarization-resolved spectroscope, and a novel three-dimensional orientational technique to understand the transport of charged dye probes interacting with the strong polyanionic brush, poly(styrene sulfonate).
Nanogels as pharmaceutical carriers: finite networks of infinite capabilities.
TLDR
This work has shown that polyelectrolyte nanogels can readily incorporate oppositely charged low-molecular-mass drugs and biomacromolecules such as oligo- and polynucleotides (siRNA, DNA) as well as proteins.
Interrogating Surface Functional Group Heterogeneity of Activated Thermoplastics Using Super-Resolution Fluorescence Microscopy.
TLDR
A novel approach for characterizing surfaces utilizing super-resolution fluorescence microscopy with subdiffraction limit spatial resolution is presented to demonstrate the effects of surface functional group heterogeneity on the electrokinetic transport process.
Supramolecular Hydrogelators and Hydrogels: From Soft Matter to Molecular Biomaterials
TLDR
This review focuses on various potential applications of supramolecular hydrogels as molecular biomaterials, classified by their applications in cell cultures, tissue engineering, cell behavior, imaging, and unique applications of hydrogelators.
...
...