Imaging Intracellular Fluorescent Proteins at Nanometer Resolution

@article{Betzig2006ImagingIF,
  title={Imaging Intracellular Fluorescent Proteins at Nanometer Resolution},
  author={Eric Betzig and George H. Patterson and Rachid Sougrat and O. Wolf Lindwasser and Scott G. Olenych and Juan S. Bonifacino and Michael W. Davidson and Jennifer Lippincott-Schwartz and Harald F. Hess},
  journal={Science},
  year={2006},
  volume={313},
  pages={1642 - 1645}
}
We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to ∼2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method—termed photoactivated localization microscopy—to image specific target proteins in thin sections of lysosomes and mitochondria; in… 
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