Imaging Golgi Outposts in Fixed and Living Neurons.


Here we describe the use of confocal microscopy in combination with antibodies specific to Golgi proteins to visualize dendritic Golgi outposts (GOPs) in cultured hippocampal pyramidal neurons. We also describe the use of spinning disk confocal microscopy, in combination with ectopically expressed glycosyltransferases fused to GFP variants, to visualize… (More)
DOI: 10.1007/978-1-4939-6463-5_3


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