Imaging FRET between spectrally similar GFP molecules in single cells

@article{Harpur2001ImagingFB,
  title={Imaging FRET between spectrally similar GFP molecules in single cells},
  author={Ailsa G. Harpur and Fred Silvester Wouters and Philippe I. H. Bastiaens},
  journal={Nature Biotechnology},
  year={2001},
  volume={19},
  pages={167-169}
}
Fluorescence resonance energy transfer (FRET) detection in fusion constructs consisting of green fluorescent protein (GFP) variants linked by a sequence that changes conformation upon modification by enzymes or binding of ligands has enabled detection of physiological processes such as Ca2+ ion release, and protease and kinase activity. Current FRET microscopy techniques are limited to the use of spectrally distinct GFPs such as blue or cyan donors in combination with green or yellow acceptors… CONTINUE READING