Imaging Bacterial Colonies and Phage–Bacterium Interaction at Sub‐Nanometer Resolution Using Helium‐Ion Microscopy

@article{Leppnen2017ImagingBC,
  title={Imaging Bacterial Colonies and Phage–Bacterium Interaction at Sub‐Nanometer Resolution Using Helium‐Ion Microscopy},
  author={M. Lepp{\"a}nen and Lotta-Riina Sundberg and E. Laanto and Gabriel Magno de Freitas Almeida and Petri Papponen and I. Maasilta},
  journal={Advanced Biosystems},
  year={2017},
  volume={1}
}
Imaging of microbial interactions has so far been based on well‐established electron microscopy methods. This study presents a new way to study bacterial colonies and interactions between bacteria and their viruses, bacteriophages (phages), in situ on agar plates using helium ion microscopy (HIM). In biological imaging, HIM has advantages over traditional scanning electron microscopy with its sub‐nanometer resolution, increased surface sensitivity, and the possibility to image nonconductive… Expand
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