Imaging Bacterial Colonies and Phage–Bacterium Interaction at Sub‐Nanometer Resolution Using Helium‐Ion Microscopy

  title={Imaging Bacterial Colonies and Phage–Bacterium Interaction at Sub‐Nanometer Resolution Using Helium‐Ion Microscopy},
  author={Miika Lepp{\"a}nen and Lotta‐Riina Sundberg and Elina Laanto and Gabriel Magno de Freitas Almeida and Petri Papponen and Ilari J. Maasilta},
  journal={Advanced Biosystems},
Imaging of microbial interactions has so far been based on well‐established electron microscopy methods. This study presents a new way to study bacterial colonies and interactions between bacteria and their viruses, bacteriophages (phages), in situ on agar plates using helium ion microscopy (HIM). In biological imaging, HIM has advantages over traditional scanning electron microscopy with its sub‐nanometer resolution, increased surface sensitivity, and the possibility to image nonconductive… 

Resolving Bio-Nano Interactions of E. coli Bacteria-Dragonfly Wing Interface with Helium Ion and 3D-Structured Illumination Microscopy to Understand Bacterial Death on Nanotopography.

In situ characterization of bacteria-nanopillar interactions in real-time close to its natural state using helium ion microscopy and 3D structured illumination microscopy techniques to understand the interaction of Gram-negative bacterial membranes with nanopillars such as those found on dragonfly wings.

Imaging Techniques for Detecting Prokaryotic Viruses in Environmental Samples

The current state of the art of imaging techniques available for characterizing viruses in environmental samples are summarized and potential links between viral imaging and environmental genomics for shedding light on the morphology of uncultivated viruses and their lifestyles in Earth’s ecosystems are discussed.

Have an Ion on It: The Life‐Cycle of Bdellovibrio bacteriovorus Viewed by Helium‐Ion Microscopy

The high resolution, the large depth of focus, and surface sensitivity of HIM hold promise to expand future studies on so far neglected ecological interactions within the microbial food web, in particular in samples with pronounced topography such as bacterial biofilms.

Bacteriophage-mediated identification of bacteria using photoacoustic flow cytometry

It is demonstrated that E. coli can be detected and discriminated from Salmonella using this method, and it is hoped that this technology will decrease the time required to identify bacterial species from 3 to 4 days to less than 1 hour.

Bacteriophage Adherence to Mucus Mediates Preventive Protection against Pathogenic Bacteria

Phages bind both to porcine mucus and to rainbow trout (Oncorhynchus mykiss) primary mucus, persist up to 7 days in the mucosa, and provide protection against Flavobacterium columnare, and exposure to mucus changes the bacterial phenotype by increasing bacterial virulence and susceptibility to phage infections.

Highest resolution chemical imaging based on secondary ion mass spectrometry performed on the helium ion microscope

This paper is a review on the combination between Helium Ion Microscopy (HIM) and Secondary Ion Mass Spectrometry (SIMS), which is a recently developed technique that is of particular relevance in

Hochaufgelöste Mikrobenporträts mit hoher Tiefenschärfe

  • Matthias Schmidt
  • Biology
    Biospektrum : Zeitschrift der Gesellschaft fur Biologishe Chemie (GBCH) und der Vereinigung fur Allgemeine und Angewandte Mikrobiologie
  • 2022
HIM is compared with electron microscopy techniques and selected examples that demonstrate the new possibilities for microbiology opened up by this technique are highlighted.

Is the Ne operation of the helium ion microscope suitable for electron backscatter diffraction sample preparation?

  • A. Wolff
  • Materials Science
    Beilstein journal of nanotechnology
  • 2021
Measurements and Monte Carlo simulations indicate that when this steady-state condition of ion impurity concentration and number of induced defects is reached more quickly, then the overall damage to the specimen is reduced.

Helium-ion-beam nanofabrication: extreme processes and applications

Helium ion beam (HIB) technology plays an important role in the extreme fields of nanofabrication. This paper reviews the latest developments in HIB technology as well as its extreme processing



Atomic force microscopy investigation of phage infection of bacteria.

Atomic force microscopy was used to study the process of infection of bacterial cells by bacteriophages, and penetration of phages inside obligate aerobe Bacillus thuringiensis was shown to be oxygen-dependent and required aeration in laboratory conditions.

Bacteriophage electron microscopy.

Conditions critical for optimal visualization of bacteriophage adsorbed to bacterial surfaces by scanning electron microscopy

Scanning electron microscopy is capable of resolving viral particles on cell surfaces, but detection of these particles is completely dependent both on the method of fixation and on the technique of drying used.

Helium Ion Microscopy (HIM) for the imaging of biological samples at sub-nanometer resolution

The advantages of the Helium Ion Microscope for imaging biological surfaces as well as compare and contrast the effects of sample preparation techniques and their consequences on sub-nanometer ultrastructure are demonstrated.

Focused ion beams in biology

An in-depth primer to the application of focused ion beams in biology, including a guide to the practical aspects of using the technology, as well as selected examples of its contribution to the generation of new insights into subcellular architecture and mechanisms underlying host-pathogen interactions are provided.

Helium Ion Microscopy Visualizes Lipid Nanodomains in Mammalian Cells.

It is shown that HIM is capable to image biological specimens without any conductive coating and that HIM images clearly allow the identification of nanodomains in the ultrastructure of membranes with 1.5 nm resolution.

Imaging of human colon cancer cells using He‐Ion scanning microscopy

A Carl Zeiss Orion Plus helium‐ion microscope is used to study the effect of sample gold coating on the morphology of human colorectal adenocarcinoma Caco2 cells and a comparative study with standard scanning electron microscopy is presented.

Dissecting Virus Infectious Cycles by Cryo-Electron Microscopy

Cryo-EM enables one to gain insight into viruses over many levels of structural hierarchy, ranging from determination of near-atomic resolution structures of subunits and symmetrical virus assemblies to ultrastructural analysis.

High Resolution Helium Ion Scanning Microscopy of the Rat Kidney

Helium ion scanning microscopy is a novel imaging technology with the potential to provide sub-nanometer resolution images of uncoated biological tissues. So far, however, it has been used mainly in