Image thresholding techniques for localization of sub-resolution fluorescent biomarkers.

  title={Image thresholding techniques for localization of sub-resolution fluorescent biomarkers.},
  author={Julien Ghaye and Madhura Avinash Kamat and Linda Corbino-Giunta and Paolo Silacci and Guy Vergeres and Giovanni De Micheli and Sandro Carrara},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  volume={83 11},
In this article, we explore adaptive global and local segmentation techniques for a lab-on-chip nutrition monitoring system (NutriChip). The experimental setup consists of Caco-2 intestinal cells that can be artificially stimulated to trigger an immune response. The eventual response is optically monitored using immunofluoresence techniques targeting toll-like receptor 2 (TLR2). Two problems of interest need to be addressed by means of image processing. First, a new cell sample must be properly… CONTINUE READING
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