Image formation model of a 3D translucent object observed in light microscopy

In this paper, we propose a complete model for the image formation of 3D microscopic translucent object. This model is essentially composed of 3 steps. The first step is the modeling of an object. We define a 3D distribution of complex indexes of refraction. The second step is based on ray tracing and simulates the light propagation inside the specimen. We… CONTINUE READING